Synthesis of monodispersed silver nanoparticles by Rhizopus Stolonifer and its antibacterial activity against MDR strains of Pseudomonas Aeruginosa from burnt patients
Author(s)- Afreen, Rathod V, Ranganath E
ABSTRACT
The extracellular synthesis of silver nanoparticles by Rhizopus stolonifer and its efficacy against multidrug resistant (MDR) strains isolated from burnt cases from hospitals at Gulbarga region, Karnataka, India is reported here. Synthesis of silver nanoparticles was carried out using fungal filtrate and an aqueous solution of silver nitrate (AgNO3). Characterization of silver nanoparticles was done by UV-Visible absorption spectroscopy shows maximum absorption at 422 nm, Transmission Electron Microscope (TEM) revealed the formation of spherical nanoparticles with size ranging between 5 to 50 nm. Energy Dispersive Spectroscope (EDS) shows the optical absorption peak at 3kev, Fourier Transform Infrared (FT-IR) shows the bands at 1633, 1554 and 1423 cm-1 which confirms the presence of protein in the sample which coat covering the silver nanoparticles known as capping proteins, and Atomic Force Microscope (AFM) revealed three dimensional structures of the nanoparticles. Two MDR-strains of Pseudomonas aeruginosa (P1and P2) from burnt patients were selected for the antibacterial study with AgNPs.